Programa de Pós-Graduação em Genética e Biologia Molecular (PPGGBM)
Multi-user Laboratories Multi-user Laboratories
CARIOLAB – Cytogenetic Analysis Laboratory, located in the Department of Genetics/CB, has a climate-controlled environment and is equipped with three multi-user devices described below, which allow for the performance of human and other organism karyotyping analyses, as well as fluorescence analyses:
- Leica DM2500 Manual Microscope – Karyo, trinocular with transmitted LED illumination for bright field and reflected illumination with Photo Fluor LM75 for fluorescence. The equipment is coupled to a Leica Cytovision capture platform, which includes a karyotyping system and a fluorescence system for fluorescence in situ hybridization (FISH), including software, computer, and camera (acquired through the Facepe Multi-User Call for Proposals 2019, Process APQ-0390-2.02/19);
- Trinocular microscope with a photography system, epi-fluorescence, and phase contrast, Leica brand, model DMLB – HBO-50W illumination with DFC340FX camera + CW4000 software;
- Trinocular biological microscope with fluorescence, Leitz brand, Model Dialux 20 EB.
The Multi-User Laboratory of the Proteomics Platform is located at the Aggeu Magalhães Institute, Fiocruz, and is equipped with instruments dedicated to the immunological and biophysical characterization of biomolecules, including:
- High-performance liquid chromatography equipment;
- Spectrophotometer;
- Circular dichroism spectropolarimeter;
- Multimodal microplate reader;
- Automated microplate washers;
- Micro-scale thermophoresis equipment.
Students, faculty, and collaborators are encouraged to contact Prof. Isabelle F. T. Viana (isabelle.viana@fiocruz.br) to schedule training and equipment usage.
The Multi-User Genomics and Transcriptomics Platform at UFPE (MULTISEQ) is equipped with tools for generating and analyzing data derived from nucleotide sequencing, including Sanger and next-generation methods. It is the academic unit responsible for providing genetic linkage determination services at UFPE, maintaining an agreement with the Public Prosecutor's Office of the State of Pernambuco to carry out paternity, maternity, and genetic profile reconstruction tests. This service is one of the most important extension activities at UFPE, with significant social relevance, in addition to enabling the learning of faculty, students, and technicians involved, contributing to the consolidation of UFPE as a regional reference in forensic genetics and human population genetics. In the context of the pandemic, it represented UFPE in the national effort for genomic sequencing of SARS-CoV-2. The genomic sequences of hundreds of circulating strains of the new coronavirus in Pernambuco were generated, contributing to the understanding of the transmission dynamics of COVID-19.